1. Product Overview

Tru Omics multi-omics service provides comprehensive biological sample analysis solutions, covering multiple fields such as genomics, transcriptomics, exomes, proteomics and metabolomics. These services are designed to help researchers gain a deeper understanding of complex biological systems and accelerate the study of disease mechanisms, drug development and personalized medicine applications. Tru Omics uses high-throughput sequencing and advanced bioinformatics analysis technology to ensure the high quality and reliability of data.

2. Product features

2.1 High-throughput sequencing technology

·  RNA-seq : comprehensively analyze gene expression profiles and reveal transcriptional regulatory mechanisms.

·  WES (Whole Exome Sequencing) : Focus on coding region variations and efficiently detect disease-causing mutations.

·  WGS (Whole Genome Sequencing) : Comprehensive coverage of the genome to discover rare variants and structural variations.

·  Proteomics : High-resolution mass spectrometry technology for comprehensive analysis of protein expression and modification.

·  Metabolomics : High-performance liquid chromatography -mass spectrometry technology to comprehensively analyze metabolite spectra.

2.2 High-quality data

·  Strict quality control : From sample processing to data analysis, every step is subject to strict quality control to ensure the accuracy and reliability of the data.

·  Standardized operating procedures : Adopt international standard operating procedures and specifications to ensure the consistency and repeatability of experiments.

2.3 Advanced bioinformatics analysis

·  Customized analysis process : Provide personalized analysis solutions according to customer needs, from data preprocessing to final report generation.

·  Powerful statistical methods : Use advanced statistical methods and machine learning algorithms to mine potential information in the data.

·  Interactive visualization tools : Provides rich visualization tools to help researchers understand and interpret data intuitively.

3. Technical principles

3.1 RNA-seq

·  Principle : Comprehensive detection of RNA molecules in cells or tissues, including mRNA, lncRNA, miRNA, etc., through high-throughput sequencing technology .

·  Applications : Gene expression profiling analysis, transcript variation detection, splicing event analysis, non-coding RNA research, etc.

·  step :

i.  RNA extraction : Extract total RNA from the sample .

ii.  Library construction : Construct a cDNA library through reverse transcription and PCR amplification.

iii.  Sequencing : Sequencing is performed using a high-throughput sequencing platform.

iv.  Data analysis : compare to the reference genome, perform gene expression quantification, differential expression analysis, etc.

3.2 WES (Whole Exome Sequencing)

·  Principle : By capturing exon regions in the genome, mutations in coding regions can be detected intensively.

·  Applications : genetic disease diagnosis, cancer genomics, functional gene research, etc.

·  step :

i.  DNA extraction : Extract genomic DNA from the sample .

ii.  Exon capture : Use specific probes to capture exon regions.

iii.  Library construction : Construct the captured DNA library.

iv.  Sequencing : Sequencing is performed using a high-throughput sequencing platform.

v.  Data analysis : Compare to the reference genome for variant detection and annotation.

3.3 WGS (Whole Genome Sequencing)

·  Principle : High-coverage sequencing of the entire genome to detect all types of genetic variation.

·  Applications : Complex disease research, population genetics, evolutionary biology, etc.

·  step :

i.  DNA extraction : Extract genomic DNA from the sample .

ii.  Library construction : Construct a genomic DNA library.

iii.  Sequencing : Sequencing is performed using a high-throughput sequencing platform.

iv.  Data analysis : compare to the reference genome, perform mutation detection, structural variation analysis, etc.

3.4 Proteomics

·  Principle : Comprehensive detection of proteins in cells or tissues through mass spectrometry technology, including expression levels and modification status.

·  Applications : protein expression profiling analysis, protein interaction research, disease marker discovery, etc.

·  step :

i.  Protein extraction : extract total protein from the sample.

ii.  Enzymatic hydrolysis : Use protease to enzymatically hydrolyze proteins into peptide fragments.

iii.  Mass spectrometry : Use high-resolution mass spectrometry for peptide identification and quantification.

iv.  Data analysis : Compare to the protein database for protein identification and quantitative analysis.

3.5 Metabolomics

·  Principle : Comprehensive detection of metabolites in cells or tissues through high-performance liquid chromatography -mass spectrometry technology.

·  Applications : Metabolic pathway analysis, disease marker discovery, drug metabolism research, etc.

·  step :

i.  Metabolite extraction : Extract metabolites from the sample.

ii.  Chromatographic separation : Use high performance liquid chromatography to separate metabolites.

iii.  Mass spectrometry : Metabolite identification and quantification using mass spectrometry.

iv.  Data analysis : Compare to the metabolite database for metabolite identification and quantitative analysis.

4. Sample requirements and testing procedures

4.1 Sample requirements

·  RNA-seq :

oAnimal  tissue : 50mg tissue sample, quick frozen in liquid nitrogen.

oCells  : 10^6-5*10^6 cells, rinsed twice with pre-cooled PBS, and quick-frozen in liquid nitrogen .

oWhole  blood : 500μL EDTA anticoagulated whole blood, short-term storage at 4℃ or long-term storage at -20℃.

o  FFPE samples : 10-15 FFPE wax blocks or 5μm FFPE wax rolls, stored at room temperature.

·  WES/WGS :

oAnimal  tissue : 50mg tissue sample, quick frozen in liquid nitrogen.

oCells  : 10^6-5*10^6 cells, rinsed twice with pre-cooled PBS, and quick-frozen in liquid nitrogen .

oWhole  blood : 500μL EDTA anticoagulated whole blood, short-term storage at 4℃ or long-term storage at -20℃.

o  FFPE samples : 10-15 FFPE wax blocks or 5μm FFPE wax rolls, stored at room temperature.

·  Proteomics :

oAnimal  tissue : 50mg tissue sample, quick frozen in liquid nitrogen.

oCells  : 10^6-5*10^6 cells, rinsed twice with pre-cooled PBS, and quick-frozen in liquid nitrogen .

oWhole  blood : 500μL EDTA anticoagulated whole blood, short-term storage at 4℃ or long-term storage at -20℃.

·  Metabolomics :

oAnimal  tissue : 50mg tissue sample, quick frozen in liquid nitrogen.

oCells  : 10^6-5*10^6 cells, rinsed twice with pre-cooled PBS, and quick-frozen in liquid nitrogen .

oWhole  blood : 500μL EDTA anticoagulated whole blood, short-term storage at 4℃ or long-term storage at -20℃.

4.2 Testing process

1.  Nucleic acid /protein/metabolite extraction and quality inspection : ensure that the purity and concentration of the extract meet the requirements.

2.  Library construction and quality inspection : Using the automated library construction workstation, 96 samples can be operated at the same time.

3.  Sequencing /mass spectrometry analysis : high-throughput sequencing platform or high-resolution mass spectrometer for analysis.

4.  Data analysis and reporting : standardized data analysis, customized data analysis, and generation of detailed reports.

5. Application scenarios

·  Research on disease mechanisms : Reveal the molecular mechanisms of diseases and discover new biomarkers.

·  Drug development : Accelerate drug screening and target verification, and improve drug research and development efficiency.

·  Personalized medicine : Support precision medicine and develop individualized treatment plans.

·  Basic research : Support basic research in the fields of cell biology, genetics, metabolism and other fields.

6. Conclusion

Through its comprehensive analysis capabilities and high-quality data, Tru Omics multi-omics services provide researchers with powerful tools to help them deeply understand complex biological systems and accelerate the progress of scientific research and clinical applications. Whether it is genomics, transcriptomics, exomes, proteomics or metabolomics, Tru Omics can provide a one-stop solution to ensure the accuracy and reliability of research.